Carbapenem Resistance in Clinical Isolates of Enterobacteriaceae : A Global Health Concern

Abstract

Introduction: Carbapenem-resistant Enterobacteriaceae (CRE) has gradually emerged and is one of the serious public health concerns worldwide. Aim: To detect Carbapenem Resistance in clinical isolates of Enterobacteriaceae and Carbapenamase production by performing Modified Hodge Test (MHT) and Combined Disc Test (CDT). Material & Methods: Identification of Isolates was done by standard bacteriological techniques. The isolates were screened for carbapenem resistance by Kirby-bauer disc diffusion method using Ertapenem as per CLSI recommendation. Detection of carbapenemase production was done by Modified Hodge test and Combined Disc test. Result: A total of 931 clinical isolates of Enterobacteriaceace were obtained from various clinical samples. Out of which isolates of Escherichia coli were 295 (31.68%). All these isolates were screened for Carbapenem resistance..Out of 931 isolates, 710 (76.26%) isolates were carbapenem screen positive. Maximum carbapenem resistance was seen in Klebsiella pneumoniae, 307 (43.23 %). Out of 710 carbapenem resistant isolates, 567 (79.85%) were carbapenemase producers.  Conclusion: Early detection, isolation and contact precaution for CRE patient will to prevent rapid dissemination of CRE infection.

Keywords: Carbapenem resistance, Enterobacteriacae, Modified Hodge Test.