Antibiotic sensitivity pattern of bacterial isolates recovered from clinical samples at tertiary care hospital in western UP, India

Abstract

Aim: The aim of this study was to demonstrate various bacteria recovered in clinical samples collected from in-patient department (IPD) and out-patient department (OPD) of the hospital and their antibiotic sensitivity pattern.Material and Methods: A total of 232 bacterial isolates were recovered in samples from urine, blood, pus & fluids and respiratory tract. The isolates were first identified by standard biochemical techniquesand then subjected to antibiotic susceptibility testing by Kirby Bauer disc diffusion method on Mueller Hinton agar plate as per CLSI guidelines.Result: Majority of bacterial isolates were recovered in clinical samples collected from IPD (66.38%). E. coli (34.05%) was the predominant isolate in the study. E. coli (59.01%) was the predominant bacteria in urine samples and was highly sensitive to colistin (100.00%) and least sensitive to ampicillin (08.33%). Coagulase negative staphylococci (45.24%), were the predominant bacteria in blood samples and maximally sensitive to linezolid (89.47%) while all isolates were resistant to penicillin. Staphylococcus aureus (25.64%) was the predominant bacteria in pus & fluids samples and was maximally sensitive to linezolid while half the isolates were methicillin-resistant staphylococcus aureus (MRSA). Acinetobacterbaumannii (44.83%) was the predominant bacteria in respiratory samples and was maximally sensitive to colistin (100.00%) while all isolates were resistant to carbapenems. Conclusion: Our study shows that E. coli was the major bacterial isolate from all clinical samples and was the predominant bacteria in urine samples. The most effective antibiotics in our study was colistin and carbapenems for gram-negative bacterial isolates and nitrofurantoin for urinary isolates of E. coli while linezolid &glycopeptide antibiotics were the most effective antibiotics for gram-positive cocci.