Bacteriological Profile and Antimicrobial Susceptibility Pattern of Blood Culture Isolates Among Septicemia Suspected Children at PMCH Patna

Abstract

Background: Bacterial infections remain an important cause of pediatric mortality and morbidity. It might be possible to reduce this by early diagnosis and proper management. When the body gets an infection, the immune system fights it. Sepsis happens when the immune system goes into overdrive and attacks the body’s own organs and tissues. Aim:Bacteriological Profile and Antimicrobial Susceptibility Pattern of Blood Culture Isolates Among Septicemia Suspected Children at PMCH Patna.Material and methods: Blood culture reports were studied in 424 suspected septicemias in patients attending the pediatrics department, using the standard technique of Mackie and McCartney. The antibiotic sensitivity was performed by Kirby-Bauer’s disc diffusion method. Risk factors for the sepsis were registered. Collected samples were processed at Department of Microbiology, PMCH, Patna.Result:Culture positivity was seen in 106 (25%) samples, and 317 (75%) samples were sterile. Gram-negative bacilli (GNB) were most common bacteria isolated 71%, followed by Gram-positive cocci (GPC) which comprises 29%, E. coli was the most commonly isolated 41.5% followed by Staph aureus 28.3%, followed by Klebsiella species 13.2%. Another organism isolated were Pseudomonas species 7.5%, Enterobacter 4.7%, Coagulase negative Staphylococcus 1.8%, Citrobacter 1.8% and Acinotobacter 0.9%. E Coli was the most common bacterial isolate in all the age groups of the study. Levofloxacin, linezolid, Ofloxacin, Vancomycin, Ampicillin &Amoxyclav were the most effective drugs for treating septicemia. In this study, it was seen that GPC were 100% sensitive to vancomycin and linezolid.Conclusion: Bloodstream infection is a challenging problem, and sometimes, it may be life threatening; therefore, timely detection, identification, and antimicrobial susceptibility testing of blood-borne pathogens are one of the most important functions of a diagnostic microbiology laboratory.