A Retrospective Study of Bacteriological Analysis of Bile Culture in Cholecystectomy Patients

Abstract

Introduction:The daily excretion of bile helps to flush out microorganisms entering the biliary tract in healthy individuals. The obstruction is due to gallstones in 80% of cases.Cholecystectomy is currently a frequently performed operation. The presence of gallstones within either the gallbladder or biliary tree is associated with the bacterial colonization of the bile.Materials and Methods:About 5 ml bile was aspirated from all patients, this collected bile from gallbladder before cholecystectomy was transported to the laboratory in sterile test-tube. The bile samples were received in the Bacteriology laboratory in liquid medium in blood culture bottles. The bottles were incubated for 24 hrs at 37C. The next day samples were inoculated on Blood agar and MacConkey agar and incubated overnight at 370C aerobically. All plates were examined for visible growth. The colonies were identified as per the standard microbiological procedure. Antibiotic sensitivity testing of the microorganisms was done by modified Kirby Bauer Disc Diffusion method on Muller Hinton agar. The results were interpreted as was recommended by the CLSI (Clinical Laboratory Standards Institute) Guidelines. Statistical Package for Social Science (SPSS) software, version 16, was used for data analysis. Results: Out of the 200 bile samples received in the Microbiology laboratory for analysis, 81 (26.47%) were culture positive. Escherichia coli with 30(37.03%) isolates were predominant, followed by Pseudomonas sp. 24(29.62%), Klebsiella sp. 12(14.81%), and Citrobacter sp. 12(11.11%). Also, 3(3.70%) isolate each of Staphylococcus aureusand Coagulase Negative Staphylococcus(CONS)was obtained. Conclusion:Therefore, it is very important to determine responsible pathogens and their antibiotic susceptibility pattern for the successful treatment of bile duct infections. This will help in better management in order to prevent morbidity and mortality among patients.