Histology and Histochemical study of Human Brunner’s glands

Abstract

Background: Brunner’s glands are the branched tubuloalveolar glands, whose secretory portions resemble mucous acini. As their name implies they are limited to the submucosa of duodenum. Objective: Histology and Histochemical study of Human Brunner’s glands. Methods: Samples were taken from duodenum of human from dissected fresh specimens. The samples were washed in normal saline, fixed in 2% calcium acetate in 10% formalin. The tissues were routinely processed and paraffin blocks were prepared. Results: The H & E preparation showed the secreting cells of Brunner’s glands of human is typically mucous in nature. The Brunner’s glands of human duodenum secretes neutral mucusubstances. Conclusion: The neutral mucosubstance is the predominant type in human Brunner’s glands. Goblet cells in human secrete acid mucin (sulphomucin).