Immuno-Histochemical Study of p63 Expression in Prostatic Lesions

Abstract

Introduction: Diseases of Prostate gland are responsible for significant morbidity and mortality among adult males all over the world. This study is carried out to study the usefulness of immuno-histochemistry using p63 basal-cell marker in prostatic lesions, especially the morphologically ambiguous ones. Aims: i) To study histopathological features of prostatic lesions. ii) To study expression of p63 basal-cell marker in prostatic lesions. iii) To correlate p63 expression with histopathology. Materials and methods: A descriptive observational study was conducted at Mahadevappa Rampure Medical College, Kalaburagi, Karnataka, India from 1st July 2017 to 30th June 2020. Specimens were sent for routine histopathological analysis followed by immuno-histochemistry analysis with p63. Statistical analysis used: Chi Square test was used for qualitative data analysis and Receiver Operator Curve was used for dichotomous data. Mean with Standard Deviation was used for quantitative data analysis. The results were considered statistically significant when p value was <0.05. Results: Of 52 specimens, the age of patients ranged from 60 to 88 years and mean being 68.61 years. Majority of patients presented with complaint of overflow incontinence. We encountered 36 cases of Benign Prostatic Hyperplasia, 13 cases of Prostatic Adenocarcinoma & 3 cases of Prostatic Intra-Epithelial Neoplasia. The ability of p63 to distinguish between Prostatic Carcinoma and non-Carcinomatous Prostatic lesions was statistically significant. Diagnostic Accuracy of p63 was 96.15%. Conclusion: Ability of p63 to distinguish between Prostatic Carcinoma and non-Carcinomatous Prostatic lesions was statistically significant. It helps in distinguishing morphologically ambiguous lesions of the prostate into benign or malignant lesions.