Clinical and serological evaluation of gastrointestinal profile by line immunoassay (LIA) in a group of patients attending tertiary care hospital of Bihar: An observational study

Abstract

Objectives: the evaluation of Clinical and serological auto-antibodies against gastrointestinal tract by gastrointestinal profile using LIA in a group of patients who have attended AIIMS Patna for symptoms suggestive of gastrointestinal disorder. Material and methods:This was a retrospective study on all samples (130-150) received for gastrointestinal profile to be done in Biochemistry central lab for a study period of May 2019 to January 2020(9 months). A total of approximately 50 samples positive or equivocal for antibodies against IF, Gliadin, ASCA, PCA and tTg antigen were analysed further for clinical data.  Results: In our study 56% and 44% female were participated most of the patients was 30-40 years followed by 20-30 years .The most common symptom Weight loss 48% was found in our study and  followed by Chronic/intermittent diarrhea 46%, weakness 24%, Abdominal pain 20%,constipation 18%, Abdominal discomfort and vomiting.We found 32% patients had below 11micromol/L iron and 54% patients had below 12gm/100ml Hb means suffring from anaemia.The ASCA positive status was a predictor for Crohn s disease(CD). in our study out of 50 patients 20% was ASCA+,2% ASCA++,4% ASCA+++and 4% ASCAwas positive. In our study on the basis of the histological diagnosis, PCA+ patients were14%, PCA++ patients 6%, PCA+++patients 12% and PCA Equivocal 4% was found.Tissue transglutaminase (tTG) has been identified as the autoantigen in CD. Out of 50 patients 28% tTG +,2% tTG ++,12% tTG +++,2% tTG Equivocal was found. Conclusion: The evaluation of Clinical and serological auto-antibodies against gastrointestinal tract by gastrointestinal profile using LIA was beneficial for identification of  the gastrointestinal diseases of the patients .

Keywords: gastrointestinal, immunoassay, clinical, patient.