The association of circulating soluble adhesive factor and markers of oxidative stress in patients of sickle cell anaemia
Keywords:
SCD (sickle cell disease), E- selectin, MDA (malonaldehyde), SOD (superoxide dismutase), Catalase.Abstract
Background:SCD is caused by a variant of the β-globin gene called sickle haemoglobin (HbS).It is one of the major condition leading to place an increasing demand on health services of India. Many prognostic and diagnostic markers have been studied so far for sickle cell disease. Objectives: To find out the relationship between circulating adhesive molecule and oxidative stress (MDA, SOD and Catalase) in sickle cell disease patients with HbAS and HbSS pattern. Material and methods: 50 pateints with HbSS pattern, 50 patients with HbAS pattern diagnosed by Hb Elecrophoresis were selected along with 50 healthy subjects with HbAA pattern (controls) were included in the study. E- selectin estimated by sandwitch ELISA and MDA, SOD and Catalase were estimated by specrophotometric chemical methods. Pearson correlation was used assess the correlation .Results:. There was significant positive correlation of MDA with E-selectin in HbAS subjects also it was found that there was significant negative correlation of SOD with E-selectin in HbAS subjects and significant negative correlation of Catalase with E-selectin in HbSS subjects.Conclusion: The study suggests that an excess of ROS certainly have implications in SCD pathophysiology, the assessment of oxidative stress in these patients may provide significant information regarding the use of current medications and may lead to new therapeutic strategies. Additional studies are needed to test the probable mechanisms involved in this complex network of markers and their role in SCD pathogenesis.