Study of storage induced Morphological and functional changes in Platelet concentrate at 22°c-24°c for 5 days

Abstract

Background and objective: Platelets are routinely isolated from whole blood and stored in plasma for 5 days. The platelets undergo various storage changes which include biochemical, structural and functional changes that occur during platelet storage under blood bank conditions. Study aimed to assess the quality of platelets in platelet concentrate and to evaluate the effects of storage on platelets in platelet concentrate. Material and methods: The study was carried out in the Department of Pathology, GMERS Medical College, Gandhinagar. The samples were collected from platelet concentrate bags under sterile conditions. Total 54 platelet samples were assessed using the quality parameters such as swirling, platelet count, WBC count, MPV, PDW and pH on day 0 and day 4 of storage. Result: A total of 54 samples were evaluated and of these 95% have fulfilled all 6 parameters of quality control. Thus in our study majority of the prepared units were of the desired quality. All the parameters were assessed and the results obtained on both the units were well above the values of recommended norms. Conclusion: The quality of platelet concentrates were well maintained within the usual 5 days storage under 22°C to 25° C thus an extension of platelet storage time is recommended.