Spectrum of organism and their sensitivity pattern in a tertiary care centre in Northern area of Bihar, India

Abstract

Objectives: This present study was to evaluate the spectrum of organism and their sensitive pattern with various antibiotics in sepsis children in a tertiary care centre of northern area of Bihar, India.  Methods: Blood for culture was collected from 426 clinically diagnosed septicemia cases following strict aseptic precautions. One milliliter (neonates) and 5 ml (children) blood was collected and inoculated into 10 and 50 ml, respectively, of brain heart infusion broth (1:10 dilution). The culture bottles were incubated at 37ºC aerobically and periodic subcultures were done onto Mac Conkey’s agar, blood agar and chocolate agar after overnight incubation on day 3, day 4 and finally on day 7 [4]. The growth obtained was identified by conventional biochemical tests and the antibiotic sensitivity testing was performed on Mueller–Hinton agar plates by Kirby– Bauer disc diffusion method. Zone diameter was measured and interpreted as per the Clinical and Laboratory Standards Institute (CLSI) guidelines [5]. Results: Most of the patients were females 228(54%) and belonged in age group of 1 years to 5 years. Major isolate organisms were E. coli 304(71.36%), P. species 170(39.91%) and S. aureus 154(36.15%). majorities of sepsis patients 304(71.36%) were infected with E.coli. Among of them, positive sensitivity of E.coli was greatly with amikacin 41(13.48%), levofloxacin 35(11.51%), meropenem 34(11.18%), netilmicin 25(8.22%), piperacillinn +tazobactam 24(7.89%) and nalidixic acid 23(7.56%). Conclusions: E. coli is the most predominant isolate organism while pseudomonas species and S. aureus are the second most common organisms in the sepsis patients in northern region of Bihar. Amikacin, levofloxacin, meropenem and nalidixic acid may be the best choice of drugs for the treatment of E. coli, S. aureus and P. species infection. 

Key words: Spectrum of organism, pattern of sensitivity, sepsis, children