Screening of pediococcusacidilactici strain one member of Lactic acid bacteria as probiotic: In vivo and vitro study

Abstract

Background: Probiotics are defined as “living microorganisms, which upon ingestion in certain numbers exert health benefits on the host beyond inherent basic nutrition”. Because of many potential health-promoting benefits, there continues to be considerable interest in the use of probiotics as biotherapeutic agents. Materials & Methods: For bacteria to exert any probiotic effect, they have to be able to survive both in stomach acid (pH 1.5) and bile acids (pH 2.5).Growth of LAB001 in presence of different concentration of bile salt Bile was observed. Salt Hydrolase (BSH) activity of LAB001 and UV-Vis spectroscopic analysis of deconjugated free bile acids was done. Test for cholesterol uptake by LAB001 and antibiotic resistance was noticed. Results: LAB001, M146 and M8 were more acid tolerant and were more bile tolerant than others. In anaerobic condition LAB001 can grow efficiently like aerobic condition. The bile salt deconjugation ability of LAB001 representing the highest BSH enzyme activity was calculated as the release of cholic acid and was 2.7 mM. Pure cholic acid and extracted free bile acid from P. acidilactici LAB001 showed a characteristic similarity in their absorption maxima. Conclusion: In conclusion, the probiotic strains isolated and characterized in this study have great potential as possible therapy for reducing cholesterol levels. The cholesterol-lowering effects of LAB001presented may be partially ascribed to BSH activity in vitro.