High fluorescence lymphocyte count in dengue
Keywords:SYSMEX-XN 1000, Dengue, High fluorescence lymphocyte count
Background: High fluorescence lymphocyte count (HFLC) is a research parameter indicating the presence of high fluorescence lymphocytes, which represent activated cells (antibody-secreting B- lymphocytes/plasma cells). HFLC in dengue fever is used to estimate the cellular immune response through the presence of blue plasma lymphocytes. Aims: To determine the HFLC in patients with dengue fever using hematology analyser, and to assess the severity of the disease based of the percentage of HFLC. Methods and Material: The present study was conducted in the department of Pathology in a tertiary care hospital. Eighty dengue positive cases and 20 controls were included in the study. HFLC values were obtained from the automated SYSMEX XN 1000 analyser. Statistical analysis of data was done by SPSS23 software. Results: Kruskal Wallis test showed significant difference in platelets between dengue fever, dengue hemorrhagic cases and control groups(p<0.01). Average HFLC% count in dengue fever group is 1.7 and in dengue hemorrhagic fever it is 18.85 and there exist significant difference between them with p<0.01. Conclusions: Increase in HFLC values can be related to the pathogenesis of dengue hemorrhagic fever. The immunological response can be detected by an increase in High Fluorescent Lymphocytes Count (HFLC) which can be observed in the automated Sysmex XN-1000 hematology analyzer. Thus HFLC can be used to assess the severity of the disease, at an early stage.
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Copyright (c) 2021 Sameeksha Alva, Purnima S Rao, Aravind Pallipady
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