In vitro antifungal susceptibility testing of ocular filamentous fungal pathogens isolated from keratitis cases
Keywords:Mycotic keratitis, Fusarium spp., Natamycin, Amphotericin B, Fluconazole.
Background: A variety of fungal species are known to cause keratitis. Filamentous fungi are often predominant cause of keratitis. As fungi belonging to different group usually differ in their pattern of susceptibility to antifungal agents commonly prescribed for mycotic keratitis, in vitro susceptibility testing usually guide ophthalmologists for selection of most appropriate agent from available antifungal armamentarium. The present study was conducted in a tertiary care teaching hospital with an aim to study antifungal susceptibility profile of ocular filamentous fungi. Material and Methods: Filamentous fungi were identified on the basis of macroscopic features of colony and microscopic characteristics. Antifungal susceptibility was performed exactly as per the methodology outlined in the Clinical and Laboratory Standards Institute (CLSI) M38-A2 document for antifungal susceptibility testing of filamentous fungi. Results: Among fungal isolates, 72 (77.4%) isolates were filamentous fungi whereas 21(22.6%) were yeasts. Fusarium spp. (43.1%) and Aspergillus spp. (29.2%) were common filamentous fungi isolated from keratitis cases. All filamentous fungi isolated from keratitis cases were susceptible to amphotericin B. Aspergillus spp. and Bipolaris spp. were resistant to natamycin. Conclusion: As inter and/or intra species variation in susceptibility to commonly used antifungal drugs do occur in ocular filamentous fungal pathogens, the present study highlights the importance of antifungal susceptibility testing of each and every isolate from mycotic keratitis cases.
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Copyright (c) 2022 Prachi Sudhir Dubal, Jyoti Anil Iravane, Ajit Shriram Damle
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